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        [KO Validated] N-Cadherin Rabbit mAb

        時間:2020-10-29 09:57   瀏覽次數:1961次

        品牌 ABclonal 產品類型 抗體
        產品名稱 [KO Validated] N-Cadherin Rabbit mAb 產品規格 50ul /100ul /200ul
        產品目錄號 A19083 產品目錄價 800/1200/2000

        背景

        This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. [provided by RefSeq, Nov 2015]

        免疫原信息

        應用

        反應物種Human, Mouse, Rat
        驗證應用WBIHCIF
        推薦稀釋比&實驗步驟
        • WB   1:500 - 1:2000  實驗步驟 ]
        • IHC   1:50 - 1:200  實驗步驟 ]
        • IF   1:50 - 1:200  實驗步驟 ]
        理論分子量140kDa
        實際分子量140kDa
        存儲緩沖液Store at -20℃. Avoid freeze / thaw cycles.
        Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
        實驗方法Western blotting    
        陽性樣本HeLa,293T,NIH/3T3,C6
        細胞定位
        純化方式Affinity purification

        [KO Validated] N-Cadherin Rabbit mAb 檢測圖片

        ABclonal:Western blot - [KO Validated] N-Cadherin Rabbit mAb (A19083) }

        Western blot - [KO Validated] N-Cadherin Rabbit mAb (A19083)

        Western blot analysis of extracts from normal (control) and N-Cadherin knockout (KO) HeLa cells, using N-Cadherin antibody (A19083) at 1:1000 dilution.
        Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
        Lysates/proteins: 25ug per lane.
        Blocking buffer: 3% nonfat dry milk in TBST.
        Detection: ECL Basic Kit (RM00020).
        Exposure time: 1min.
        ABclonal:Western blot - [KO Validated] N-Cadherin Rabbit mAb (A19083) }

        Western blot - [KO Validated] N-Cadherin Rabbit mAb (A19083)

        Western blot analysis of extracts of various cell lines, using N-Cadherin antibody (A19083) at 1:1000 dilution.
        Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
        Lysates/proteins: 25ug per lane.
        Blocking buffer: 3% nonfat dry milk in TBST.
        Detection: ECL Basic Kit (RM00020).
        Exposure time: 1min.
        ABclonal:Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083) }

        Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083)

        Immunohistochemistry of paraffin-embedded mouse heart using N-Cadherin Rabbit mAb (A19083) at dilution of 1:100 (40x lens).
        ABclonal:Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083) }

        Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083)

        Immunohistochemistry of paraffin-embedded rat heart using N-Cadherin Rabbit mAb (A19083) at dilution of 1:100 (40x lens).
        ABclonal:Immunofluorescence - [KO Validated] N-Cadherin Rabbit mAb (A19083) }

        Immunofluorescence - [KO Validated] N-Cadherin Rabbit mAb (A19083)

        Immunofluorescence analysis of mouse heart using N-Cadherin Rabbit mAb (A19083) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.