產品介紹

背景

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.
Note：Dilution method
Put 5ul AC033 into 45 ul buffer(with 50% glycerol), diluted 1:10,000 for using. The diluted antibody can be stored at -20℃ without aliquot.

免疫原信息

免疫原	Recombinant protein of human GAPDH
序列	Email for sequence
基因ID	2597
Swiss Prot	P04406
別名	G3PD;GAPD;HEL-S-162eP;GAPDH
研究領域	Endocrine & Metabolism內分泌代謝Carbohydrate metabolism碳水化合物代謝 Neuroscience神經科學Neurodegenerative Diseases神經退行性疾病Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimer's Disease阿爾茨海默病中淀粉樣斑塊與神經原纖維纏結的形成

應用

反應物種	Human, Mouse, Rat
驗證應用	WB IHC IF
用戶驗證的應用	WB (Mus musculus , Homo sapiens , Rattus norvegicus , Schisandra chinensis) IP (Mus musculus) IF (Mus musculus , Homo sapiens) DB (Homo sapiens)
推薦稀釋比&實驗步驟	WB 1:50000 - 1:200000 [ 實驗步驟 ] IHC 1:50 - 1:200 [ 實驗步驟 ] IF 1:50 - 1:200 [ 實驗步驟 ]
抗體稀釋方法	Put 5ul AC033 into 45 ul buffer(with 50% glycerol), diluted 1:10,000 for using. The diluted antibody can be stored at -20℃ without aliquot.
理論分子量	31kDa/36kDa
實際分子量	36KDa
存儲緩沖液	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
實驗方法	Western blotting
陽性樣本	HeLa,HepG2,293T,Mouse liver,Mouse kidney,Mouse spleen,Rat liver,Rat kidney
細胞定位	Cytoplasm,Membrane,Nucleus,cytoskeleton,cytosol,perinuclear region
純化方式	Affinity purification

GAPDH Mouse mAb (High Dilution) 檢測圖片

Western blot - GAPDH Mouse mAb (High Dilution) (AC033)

Western blot analysis of extracts of various cell lines, using GAPDH Monoclonal Antibody (AC033) at 1:20000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Western blot - GAPDH Mouse mAb (High Dilution) (AC033)

Western blot analysis of extracts of HeLa cells, using GAPDH antibody (AC033) at 1:10000-1:2560000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Immunohistochemistry - GAPDH Mouse mAb (High Dilution) (AC033)

Immunohistochemistry of paraffin-embedded Human esophageal using GAPDH Mouse mAb (AC033) at dilution of 1:100 (40x lens).

Immunofluorescence - GAPDH Mouse mAb (High Dilution) (AC033)

Immunofluorescence analysis of C6 cells using GAPDH Mouse mAb (AC033) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence - GAPDH Mouse mAb (High Dilution) (AC033)

Immunofluorescence analysis of NIH-3T3 cells using GAPDH Mouse mAb (AC033) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence - GAPDH Mouse mAb (High Dilution) (AC033)

Immunofluorescence analysis of U-2 OS cells using GAPDH Mouse mAb (AC033) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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